Isolation, Phenotypic and Molecular characterization of vancomycinresistant Staphylococcus aureus in a Tertiary care Hospital, in CentralIndia

Background/Aims: Staphylococcus aureus (S. aureus) is a major cause of infections both in hospitals andcommunities worldwide and has demonstrated resistance to commonly prescribed antimicrobial agents. ThisGram-positive bacterium, commonly found in the environment, is part of the natural flora of humans. Over 60%of healthy individuals carry S. aureus on their skin and mucous membranes, particularly in the upper respiratorytract.Aim: Isolation, Phenotypic and Molecular characterization of vancomycin resistant Staphylococcus aureus in aTertiary care Hospital, from Central IndiaObjectives:1. Isolation of Staphylococcus aureus from various clinical samples of the patients attending a tertiary careHospital, from Central India.2. Phenotypic characterization and antibiotic susceptibility pattern of Staphylococcus aureus using Vitek-2 au-tomated methods and microbroth dilution method.3. Molecular characterization of vancomycin resistance in Staphylococcus aureus for detection of genes likeVan A, B, D, E, and G by using multiplex polymerase chain reaction.Sample Collection and Bacterial Isolation:• A total of 150 clinical samples, including pus, blood, urine, and sputum, were collected from patients withsuspected MRSA, infections.• Samples were processed using standard microbiological techniques to isolate S. aureus.Result: A total of 150 clinical specimens were processed for this study, including 6 urine samples, 13 diabeticfoot swabs, 17 blood samples, 7 bronchoalveolar lavage (BAL) samples, 9 pleural fluid samples, 5 bronchialaspirates, 21 throat swabs, 12 biopsy samples, 3 CSF samples, 4 stool samples, 25 sputum samples, and 7 cathetertips.Conclusion: The results of the current study illustrate the emergence of vancomycin resistance among methicillin-resistant S. aureus isolated from children with healthcare-associated infections. The majority revealed theoccurrence of vanA and vanB as an accountable mechanism for this resistance.