Generation of Nonintegrative‐Induced Pluripotent Stem Cells in Hutchinson‐Gilford Progeria Syndrome: Enhancing Aging Research

Heart failure and cardiovascular disease are the primary causes of mortality in patients with Hutchinson‐Gilford Progeria Syndrome (HGPS), resulting from mutations in the LMNA gene. In this study, we investigated a familial pedigree of HGPS to derive induced pluripotent stem (iPS) cells using a nonintegrating Sendai virus method from both affected and unaffected family members. The goal was to create a comprehensive robust and reliable cellar model to study the accelerated aging disease's progression. The resulting iPSCs fulfilled all pluripotency criteria, demonstrated differentiation into all three germ layers, and retained the HGPS mutation. In addition, these iPSCs exhibited key HGPS characteristics, such as shortened telomeres, abnormal Lamin A (Progerin) expression, and reduced cardiac marker levels. Thus, our approach offers a robust, reliable, and accurate representation of the disease pathology for the aging community.