Efficacy of Ethanol Extract of Papaya Leaves (Carica papaya L. var California) on Mortality of Instar III Larvae of Aedes aegypti Mosquito

This study aims to evaluate the efficacy of Papaya var California leaves extract and identify its effective concentration in inducing mortality among third-instar larvae of Aedes aegypti mosquitoes. The study utilized adult Ae. aegypti mosquitoes as the experimental subjects. This study was an experimental study with a comparative Completely Randomized Design The test groups include negative control, 2.5 mg/mL, 5 mg/mL, 10 mg/mL and positive control. Each test used 20 third instar larvae of Ae.aegypti mosquitoes, with four repeats.. The test groups were 2.5 mg/mL, 5 mg/mL, 10 mg/mL, and the control groups were A negative control contained distilled water and Tween 20, and a positive control contained abate (temephos) Based on Kruskall-wallis test shows a significant difference. The results indicated that the optimal concentration was 10 mg/mL, with the highest effectiveness observed at the 30th time hours.