The dynamics of oxidoreductase expression in the liver during experimental peritonitis and the role of p38 MAPK inhibition

Background. Oxidative stress is crucial in the pathogenesis of endogenous intoxication syndrome in acute surgical abdominal pathology. The enzyme systems of oxidative phosphorylation and cytochrome reductase are vital for hepatocyte energy potential. This study aimed to investigate the effects of a p38 MAPK inhibitor on cytochrome b5 reductase 3 (CYB5R3); cytochrome c oxidase; and microsomal oxidation in experimental peritonitis. Methods. A model of peritonitis was used with two groups: a control group without treatment (n = 15) and a main group treated with adezmapimod conjugate on endof-surgery (n = 15). Immunomorphological staining was employed to evaluate CYB5R3 and cytochrome oxidase expression on days 3; 7; and 14. The intensity of staining was scored from 0 to 4; and differences were assessed using multiple comparison tests and the Mann-Whitney criterion. Results. In the control group; CYB5R3 intensity decreased from 2.5 [2.0;3.0] on day 3 to 1.0 [1.0;1.0] on day 14. Conversely; in the main group; the inhibitor maintained high enzyme expression throughout the study. On day 3; cytochrome oxidase levels differed significantly between groups: 1.0 [1.0;2.0] in the control and 4.0 [3.0;4.0] in the main group (p < 0.05). Conclusion. The findings suggest that adezmapimod conjugate positively affects the integrity of enzyme systems; maintaining redox balance and oxidative phosphorylation in hepatocytes during peritonitis.