Genetic profile of embryos in the assisted reproductive technology program in Kuzbass (Kemerovo) based on the results of preimplantation genetic testing

Objective : to study the frequency and structure of chromosomal abnormalities in embryos obtained as part of ART in Kuzbass women with infertility. Material and methods: 58 embryos obtained as a result of in vitro fertilization-intracytoplasmic sperm injection (IVF-ICSI) were examined for the presence of aneuploidies at the Center for Family Health and Reproduction «Krasnaya Gorka» by performing (preimplantation genetic testing for aneuploidy) PGT-A. Results: 60.3% (35) of the embryos contained chromosomal abnormalities. Of these, 77.08% of embryos had aneuploidy, 18.75% had mosaicism, and 4.17% had polyploidy. In the cohort of women whose AMH levels were reduced, 62.5% of embryos had CA. The frequency of CA embryos in women over 35 years of age is 57.1%. Analysis of the CA structure of embryos in a cohort of patients with pathospermia revealed that 57.7% of embryos were euploid. 42.31% of embryos had CA in their genetic profile. Conclusions: comparing the detected frequency of chromosomal abnormalities (CA) in embryos with the results of studies by foreign colleagues, we can say that the level of euploid, aneuploid and mosaic embryos obtained aſter PGT-A is approximately equal in different populations: 60% of embryos have CA. Among all CA, aneuploidies dominate with a frequency of 77.08%. Among aneuploidies, trisomy of chromosome 16 was prevailed (12.5%). The incidence of mosaicism was 18.75%.