DNAzyme-Driven Dynamic Cycling Biosensor with Dynamic Fluorescent and Cumulative Electrochemiluminescence Monitoring of Single-Nucleotide Variants

Nucleic acid-based constitutional dynamic networks (CDNs) can mimic biological events while revealing complex biological processes and their functionalities. In this study, we present a novel dual-signal biosensor that leverages a dissipative DNAzyme-driven network for the analysis of mutant targets. A dissipative cycle response to target in single-base resolution was designed based on a DNAzyme-driven network to generate transient fluorescence signals, enabling real-time monitoring of mutant interactions. Meanwhile, a secondary signal mechanism using bipolar electrodes for electrochemiluminescence (ECL) detection was introduced, which provided a cumulative recording of the response events. The dual mechanisms ensured the efficient and specific activation of the dissipative cycle, mimicking SNV-related biological processes and enhancing the sensitivity and selectivity. This biosensing platform demonstrates the potential in advanced analytical applications, including real-time mutant detection and bioprocess simulation, paving the way for innovative diagnostic and research tools in analytical chemistry.