A Viable Strategy for the Biodegradation of Halogenated Organic Compounds From the Wastewater Mediated by Pseudomonas Putida

Microbial degradation is to be considered a perspective solution in the elimination of microporous organic compounds in the environment. Various studies attest to the Pseudomonas putida species as being capable of aerial biodegradation of numerous organic compounds including chlorinated aromatic compounds. The aim of this study was to demonstrate the ability of Pseudomonas putida to grow on various organic substrates, being a possible candidate for dehalogenation of various chlorinated organic compounds Water samples were prepared by contamination with a solution containing 1,1,1-trichloroethane, 1,1,1,2-tetrachloroethane, trichloromethane and tetrachloromethane. The concentrations of halogenated organic compounds, before and after inoculation, were determined by gas chromatographic method. The results obtained showed the reduction, in the presence of Pseudomonas putida, of various halogenated organic compounds such as 1,1,1-trichloroethane, 1,1,1,2-tetrachloroethane, trichloromethane and tetrachloromethane with values ranging from 23% to 45% over a time interval of 120 days. The present study demonstrates the ability of Pseudomonas putida to grow on various organic substrates, being a possible candidate for dehalogenation of various types of chlorinated organic compounds.