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This is a Experimental Study study.

Standardized amino acid digestibility and nitrogen-corrected true metabolizable energy of frozen raw, freeze-dried raw, mildly cooked, and retorted dog foods using the precision-fed cecectomized and conventional rooster assays

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Research reveals amino acid digestibility differences in dog foods, suggesting processing methods significantly affect nutrient retention.

Processing methods influenced the digestibility of amino acids in dog foods, with cooked diets showing higher digestibility for certain amino acids.
The study found that the cooked diets, sous vide and steamed, had significantly higher amino acid digestibility compared to other methods.
Precision-fed rooster assays were used to evaluate amino acid digestibility and metabolizable energy content in six dog food formulations.
The results indicate that heat processing can reduce nutrient availability, highlighting the importance of choosing appropriate cooking methods for pet foods.

Geary et al. (2025) studied this question.

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Research reveals amino acid digestibility differences in dog foods, suggesting processing methods significantly affect nutrient retention.