Preformulation studies of thermosensitive dermatological hydrogels containing insulin

This work continues previous research into the development of an insulin (INS) hydrogel carrier for dermatological applications in chronic wound healing. Insulin has been found to play an important role in promoting wound healing, acting at multiple levels. This study aimed to develop an insulin hydrogel carrier based on poloxamer 407 (P407) and chitosan (CS). Rheological and texture parameters of the formulation were analysed. The pharmaceutical availability of insulin was assessed in vitro using the innovative Strat-M® membrane, which mimics the skin barrier. The hydrogels showed a favourable balance between rheological and textural properties and ease of application, especially to irregularly shaped wounds and hard-to-reach areas. Insulin was released in a prolonged manner, reducing the need for multiple daily applications. The obtained results will be used further in planning and performing preclinical and clinical studies.