Autologous Platelet Rich Plasma Preparation for Regenerative and Intra-Articular Applications

Background: Platelet-rich plasma (PRP) has been used in many regenerative and intra-articular therapies due to its regenerative probabilities with high growth factor content. However, lack of standardized protocols for preparation limits reproducibility and comparison across studies. Methods: A reproducible protocol for autologous PRP preparation with usage of a manual double-spin method was applied in ten patients. 25 ml of peripheral venous blood were drawn under aseptic condition into 5 ml of 3.8% sodium citrate as anticoagulant. The first centrifugation performed at 2300×g (gravitational acceleration) for 3 minutes to separate red blood cells. The upper plasma portion was transferred and centrifuged again at 2300×g for 8 minutes. Platelet-poor plasma was discarded, and the platelet pellet was resuspended in 3 mL of plasma. Results: The protocol consistently produced a homogeneous PRP with a platelet concentration of at least four times baseline were achieved in all preparations. Samples were successfully aspirated into sterile syringes without technical difficulty. Conclusion: The described technique provides a simple, cost-effective, and reproducible method for PRP preparation that can be performed in operating room with usage of a centrifuge and sterile empty tubes. This protocol supports consistent platelet concentration and may facilitate clinical use and intraoperative applications.