Most protein structures deposited in the Protein Data Bank have been determined at 100 K by collecting X-ray diffraction data while holding crystals under a liquid nitrogen flux. Recently, the growing awareness that low-temperature diffraction data collection is accompanied by artifacts and by a loss of physiologically relevant information has driven crystallographers to collect X-ray diffraction data at room temperature or at body temperature (37 °C). Here, the results obtained from recent structural determinations of metal-protein adducts at body temperature are briefly discussed.
Antonello Merlino (Mon,) studied this question.