PCR detected monkeypox virus clade Ib in wastewater, suggesting its presence correlates with confirmed cases.
We used a sensitive and specific PCR to detect monkeypox virus clade Ib DNA from >3,000 wastewater samples collected prospectively across the United States. Monkeypox virus clade Ib DNA was detected in 1 sample from a location with a confirmed case; it was not detected in locations with no confirmed cases.
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Boehm et al. (2025) studied this question.
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