Research on the content and activity of CYP450 enzymes in human liver

Cytochrome P450 (CYP450) enzymes play a crucial role in drug metabolism, and their content and activity are important parameters in pharmacokinetic studies. However, due to variations in sample size, background information, and detection methods across different studies, the reported results have shown considerable discrepancies. Therefore, comparative analyses of different studies are essential to obtain more objective and accurate data for pharmacokinetic research. Recent advancements in mass spectrometry techniques have facilitated the measurement of CYP450 protein content, leading to a surge of new studies and enabling more comprehensive comparisons. This review integrates our group's recent findings with relevant literature to summarize the inter-individual variability in human liver CYP450 enzyme content and activity, the changes across molecular, microsomal, hepatic tissue, whole liver, and whole-body levels, and the impact of factors such as content, genetic polymorphisms, and POR on enzyme activity. Our aim is to provide a more comprehensive comparison and reference for related research.