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This is a Quantitative Study study.

Influence of Successive Subcultures and Hormone Concentrations on Somaclonal Variation in Tissue-Culture-Derived Pineapple (Ananas comosus var. MD2)

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Investigation shows increasing hormone levels and subcultures raise somaclonal variation in pineapple, affecting fidelity.

Extended subculturing beyond 8 passages increases somaclonal variation in pineapple tissue cultures.
At the 10th subculture, abnormalities rose to 0.90%, indicating that prolonged cycles impact plant fidelity.
Higher concentrations of benzylaminopurine caused morphological changes, increasing spiny forms to 12% at 10 mg L⁻¹.
Recommendations include limiting subculture cycles and maintaining lower hormone levels to preserve clonal fidelity.

Rahman et al. (2025) studied this question.

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View Full Paper

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Authors

Investigation shows increasing hormone levels and subcultures raise somaclonal variation in pineapple, affecting fidelity.

Extended subculturing beyond 8 passages increases somaclonal variation in pineapple tissue cultures.
At the 10th subculture, abnormalities rose to 0.90%, indicating that prolonged cycles impact plant fidelity.
Higher concentrations of benzylaminopurine caused morphological changes, increasing spiny forms to 12% at 10 mg L⁻¹.
Recommendations include limiting subculture cycles and maintaining lower hormone levels to preserve clonal fidelity.

Rahman et al. (2025) studied this question.

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