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October 1, 2025Microbiology SpectrumOpen Access

Rapid and accurate sepsis diagnostics via a novel probe-based multiplex real-time PCR system

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Authors

MFMarco FavaroUniversity of Rome Tor VergataCFChristian FiniNTT (Italy)MCMaurizio CapannariNTT (Italy)

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Overview

Observational analysis evaluates diagnostic accuracy for sepsis in 228 blood cultures, indicating potential for improved patient management.

Key Points

  • The SEPSI ID panel achieved sensitivity of 96.88% and specificity of 100%, indicating strong performance.
  • Rapid diagnostics using multiplex PCR can significantly reduce turnaround time for sepsis identification.
  • Both SEPSI ID and SEPSI DR panels identified additional pathogens and resistance targets not detected conventionally.
  • The system processes just 2 µL from positive blood cultures, eliminating nucleic acid extraction for quicker results.

Cite This Study

Favaro et al. (2025) studied this question.

synapsesocial.com/papers/68dd9537fe798ba2fc499740https://doi.org/10.1128/spectrum.00559-25
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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A-375 Fast-Track Sepsis Detection: Genes2Me *Sepsis-Q* Direct Blood Test for 32 Pathogens in 120 Minutes2025
  2. 2Evaluation of the Performance of Novel Gram-Negative and Gram-Positive Sepsis Panels for the Rapid Diagnosis of Bloodstream Infections2026
  3. 3Diagnosing Sepsis and Identifying Bacterial Resistance Genes Directly from Whole Blood Using a Rapid Real-Time PCR System2026
  4. 4Fast Microbiology: A Pilot Study Comparing Culture-Independent Digital PCR and Blood Culture for Pathogen Detection in Sepsis2026
  5. 5Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis - A Targeted, Blood Culture Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients2024 · 1 citations