Assessment of the efficiency of recombinant strains-producers of Bacillus pumilus 3-19 metalloendopeptidase

Minor zinc-dependent metalloendopeptidase secreted by the soil strain Bacillus pumilus 3-19 occupies a unique intermediate classification position between two families of the metzincin clan: adamalizins and astacins. To be able to study the functional role of metalloendopeptidase in more detail, it is necessary to obtain a pure protein preparation in sufficient quantity. Since the enzyme secretion by the B. pumilus 3-19 strain is extremely low, recombinant strains producing metalloendopeptidase MprBp were constructed based on protease-free strains of Bacillus subtilis, as well as methylotrophic yeast Pichia pastoris. The obtained strains were evaluated for the efficiency of metalloendopeptidase production. The B. subtilis strain BG2036+mprBp had the highest production of the target protein.