Cellular composition of DLBCL NOS (n = 99) characterized using mIF. Cells were phenotyped using the Phenograph algorithm followed by manual thresholding of particular markers to divide unspecific Phenograph clusters. A, Representative image of a TMA core stained with mIF and a Voronoi plot of the same core with cell phenotypes labeled. B, A heatmap with mean expression values of the stained markers in each identified cell phenotype. C, A t-distributed stochastic neighbor embedding (t-SNE) plot performed with the expression of all stained markers in the segmented cells; 5% (60,938 cells) of the cells were randomly selected for plotting. D, A barplot depicting the total number of identified cells of each cell phenotype. E, A boxplot depicting the median proportions of different cell phenotypes in the DLBCL NOS samples. Lower and upper hinges of the boxplot correspond to the 25th and 75th percentiles, and whiskers the confidence intervals 1.5 × IQR from the hinge. F, A barplot depicting the cellular composition of the lymphomas in each patient in the cohort (n = 99).
Autio et al. (Wed,) studied this question.
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