Figure 5 from Spatial Analysis of the Tumor Microenvironment in Diffuse Large B-cell Lymphoma Reveals Clinically Relevant Cell Interactions and Recurrent Cellular Neighborhoods

The relation between RCNs and LME subtypes and DLBCL genetic subtypes based on LymphGen classification and driver mutations (n = 85). A, A heatmap depicting the average proportions of RCNs in the different LME subtypes. B, A boxplot depicting the median proportion of cells with an immune-poor RCN5 neighborhood in depleted (DP) DLBCLs compared with GC-like (GC), mesenchymal (MS), and inflamed (IN) DLBCLs. The groups were compared using Mann–Whitney U test. C, A boxplot depicting the median proportion of cells with a PD-L1⁺ M2-like TAM-rich RCN10 neighborhood in inflamed DLBCLs compared with GC-like, mesenchymal, and depleted DLBCLs. The groups were compared using Mann–Whitney U test. D, Oncoprint of selected mutations and proportions of RCNs in DLBCL genetic subtypes. E, A boxplot depicting the median proportion of cells with a PD-L1⁺ M2-like TAM-rich RCN10 neighborhood in different genetic subtypes. The groups were compared using Kruskal–Wallis H test. F, A boxplot depicting the median proportion of cells with a PD-L1⁺ B cell–rich RCN7 neighborhood in different genetic subtypes. The groups were compared using Kruskal–Wallis H test. G, A boxplot depicting the median proportion of cells with a B cell–rich and immune cell–rich RCN1 neighborhood in DLBCLs with and without GNA13 mutations. The groups were compared using Mann–Whitney U test. H, A boxplot depicting the median proportion of cells with a M1-like TAM-rich RCN8 neighborhood in DLBCLs with and without PRDM1 mutations. The groups were compared using Mann–Whitney U test. Lower and upper hinges of the boxplots correspond to the 25th and 75th percentiles, and whiskers to the confidence intervals 1.5 × IQR from the hinge.