SAP(1-3) Gene Expression in High Proteinase Producer Candida Species Strains Isolated from Iranian Patients with Different Candidosis

Candida species are one of the commensal organisms that produce diseases in immune deficiency or disrupted normal flora. Several virulence factors such as proteinase production have an aggressive role in Candida species. Aspartyl proteinase gene expressed some molecular proteins that have an important role in virulence of Candida species. In this study 73 isolates from outpatient of Iranian Pasteur institute from various site of origin were analyzed. These species identified up to species level by standard mycological techniques and tested for proteinase activity and SAP1-3 expression by Real-time PCR. SAP1-3 gene expression was assayed in Candida species that shown a high producer of proteinas activity and compared them with the source of infection. Our results was shown the SAP1-3 protein in vaginal C. albicans was detected in 80% and SAP2 in 100% of studied strain. In sputum all three saps detected but not in all strain. It is concluded that C. albicans have a high ability to proteinase production. This ability result in more virulence and pathogenic effect. In other hand, SAP expression in Candida species is strain and source dependent and plays an important role in candidosis infections.