Figure 4 from Combined Oxygen-Enhanced MRI and Perfusion Imaging Detect Hypoxia Modification from Banoxantrone and Atovaquone and Track Their Differential Mechanisms of Action

Banoxantrone (BN) hypoxia modification is replicated in U87, a xenograft model with different growth and hypoxia characteristics. U87 xenografts were less hypoxic than Calu6 xenografts. The group average ΔR₁ for size matched (A) Calu6 was less than that for (B) U87 xenografts. Error bars are SEM. Significant difference between (C) group ΔR₁ and (D) group MRI hypoxic volume were observed between the two tumor models. Examining the U87 xenografts further, (E) U87 Cohort reduction in MRI hypoxic fraction was seen at day 3 in treated drug tumors, relative to control tumors (Cn; white bars), with (F) U87 example segmented MRI images showing hypoxic (blue), normoxic (yellow), and necrotic (gray) tumor. G, Lower percentage of pimonidazole staining was detected in drug-treated U87 tumors at day 3, compared to controls. H, Example pimonidazole adduct formation images (×40 magnification).