Covalent labeling of Matrix Metalloproteases with affinity‐based probes containing tuned reactive N‐acyl‐N‐alkyl sulfonamide cleavable linkers

Original covalent probes with an N-acyl-N-alkyl sulfonamide cleavable linker were developed to target a broad set of human Matrix Metalloproteases (MMPs). The electrophilicity of this cleavable linker was modulated to improve the selectivity of the probes as well as reduce their unspecific reactivity in complex biological matrices. We first demonstrated that targeting the S