Extraction of Bacterial Pigments and Evaluation of its Antioxidant Activity

Carotenoid are pigment that exist in a wide variety of plant and microorganism they are characterized by red, yellow or orange coloration .These important pigment play significant role in animal and human health .In contrast to plant production, the advantages of microbial fermentation of carotenoids were lower media cost, fast growth rate of microorganism and the ease of culture control . When the microbial cells are used to produce the color the term refers to ‘microbial pigments’. Different types of pigments extracted from different microbes. In this study, soil and water samples were collected from different areas of MGM University, Chh. Sambhajinagar .Different pigmented colonies were observed on nutrient agar plate .Out of these, the orange and yellow colonies were selected and sub-cultured further to obtain their pure cultures. These samples were inoculated onto nutrient agar plates, and after an incubation period of 24 hours, different colonies were observed on the plates. Out of these, the orange and yellow isolated were selected and sub-cultured further to obtain their pure cultures. After production of the pigment in liquid medium, it was further processed for the extraction of these pigments. Further, antioxidant assay by radical scavenging activity of DPPH was carried out, which revealed that the pigment had an radical scavenging activity of 60% as compared to that of the standard ascorbic acid, which showed radical scavenging activity of 33 %. Thus, the current study can be a useful step for large-scale production of pigments, their purification and application in cosmetic industries