BMSCs Combined with Grafted igament Promote ACL Healing by Regulating NF-kB Signaling Pathway The mechanism of action of the combination

Abstract Anterior cruciate ligament (ACL) injury is one of the most common knee injuries, and there is still no agreement on the mechanism of graft healing after ligament reconstruction. In this study, a rat model of bilateral anterior cruciate ligament (ACL) injury was established, and a series of in vitro and in vivo biological and cytological methods were used to explore the mechanism of NF-kb signaling pathway regulated by BMSCs combined with transplanted ligament in the healing of ACL reconstruction ligament. Research shows that BMSCs and co cultured damaged ligament models reduce collagen fibers and inflammation; Transcriptome sequencing revealed Ptgs2, Birc3, Icam1, Tnfrsf1a, and IL-1 β The expression of factors may be regulated by BMSCs and participate in anterior cruciate ligament injury. Further validation through Western Blot, RT qPCR, and immunohistochemistry revealed that bilateral anterior cruciate ligament injury implanted with transplanted tendon reconstruction can significantly increase TNF- α、 IL-1 β、 NF- ĸ The expression levels of B and Ptgs2 can be reduced by BMSCs treatment. Regulating NF- κB The important role of the B signaling pathway in anterior cruciate ligament healing; Prove that the combination of bone marrow mesenchymal stem cells screened in vitro and xenograft ligaments can effectively promote anterior cruciate ligament healing in a rat model (animal model).