Fumonisin B 1 (FB 1 ), which is emerged by contamination of corn and similar products with Fusarium species, causes a high risk for human and animal health. FB 1 shows hepatotoxic, nephrotoxic, neurotoxic, immunotoxic and teratogenic effects. FB 1 has also been linked to carcinogenesis processes that are unrelated to genotoxic pathways. Our goal was to investigate the role of gene-specific DNA methylation and expression of tumour suppressor genes in the toxicity of FB 1 in human kidney (HK-2) cells. Using array panels, effects of FB 1 exposure (10-100 μM) for 24 h on methylation levels of tumour suppressor genes were investigated. ToppGene Suite (Toppfun) portal was used to perform gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and Reactome enrichment studies. The most of the genes were hypermethylated significantly at 100 μM concentrations of FB 1 . These results are consistent with the expression results which downregulate the selected genes of CD44 , CDKN1A , CDKN2B , CTNNB1 , MYC and p16 . Functional enrichment analysis further elucidated the molecular mechanisms of FB 1 in cancer-related pathways especially p53 signalling and PI3K/Akt signaling. It has been expected that the present study could contribute to the studies on the mechanisms that play a role in FB 1 induced carcinogenesis.
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Bayoglu et al. (2024) studied this question.
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