Detection of the prevalent biofilm-producing bacteria in hearing aids and silico stimulation of effective anti-biofilm agents

The majority of tympanoplasty surgeons recommend air-conduction hearing aids to patients who have residual hearing loss.The present study aimed to use silico stimulation against gram positive and negative bacteria isolated from hearing aids that produce biofilm formations.More than 150 patients (hearing aid users), including both sexes, were surveyed to gather samples for the study of hearing aids. From September to December 2023, the samples were collected from Ramadi Teaching Hospital and Ramadi Children and Maternity Hospital in Ramadi, Iraq . There was a 64/53.3% increase in the prevalence of harmful microorganisms in men compared to females who used hearing aids. Most infected individuals were 70 and older (25/20.8% ). The microtitration plate method was used to determine the biofilm formation ability of the isolates. The findings showed that 15% of Staphylococcus aureus isolates formed strong biofilms, 5.8% of the isolates of Psuedomonas. aeruginosa formed strong biofilms, and only 1% of the isolates of Stapylococcus. epidermidis, Escherichia coli, Proteus mirabilis and Klebsiella pneumonia formed strong biofilms. There is a According to the latest research, the risk of microbial infection in those who use hearing aids increases over time. When different protein-drug docking analyses were done, different species (P. mirabilis, Ps aeruginosa, Staphylococcus aureus, and Streptococcus pneumonia) had different binding affinities. The molecular docking analysis of all the proteins with the selected drug compounds resulted in varied binding affinities for all the species (P. mirabilis, Ps. aeruginosa, S. aureus, and St. pneumoniae). The binding affinities for all species resulted in the range from -13.52 kcal/mol (aliB-Cefilavancin complex) to -2.72 kcal/mol (fib-Disulfiram complex). The ear canal's microbiota changes when a hearing aid (HA) is worn. It raises the risk of otitis externa by promoting the growth of pathogenic bacteria.