What question did this study set out to answer?

To determine whether modifying biomaterial surface charge prevents Pseudomonas aeruginosa biofilm attachment in vitro and decreases bacterial persistence on titanium implants in vivo.

March 14, 2017The Laryngoscope

Surface charge modification decreases Pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model

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Authors

WKW. Katherine KaoThe University of Texas Health Science Center at Houston PGPatricia M. GagnonWashington University in St. Louis JVJoseph P. VogelWashington University in St. Louis

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Implication

Preclinical study reveals surface charge modification decreases Pseudomonas aeruginosa adherence and implant colonization in rats, suggesting a strategy to prevent device infections.

Key Points

To determine whether modifying biomaterial surface charge prevents Pseudomonas aeruginosa biofilm attachment in vitro and decreases bacterial persistence on titanium implants in vivo.
Modified cell culture wells using layer-by-layer polyelectrolyte assembly to evaluate Pseudomonas aeruginosa adherence at 30-, 60-, and 120-minute intervals in vitro.
Surgically implanted charge-modified and unmodified titanium microscrews into the dorsal calvaria of adult rats, inoculated them with P. aeruginosa, and evaluated bacterial burden after 2 weeks.
Surface charge modification led to a statistically significant decrease in bacterial adherence across all evaluated time points in vitro.
Charge-modified titanium implants showed a statistically significant reduction in persistent P. aeruginosa recovered 2 weeks after surgical implantation and inoculation.

Cite This Study

Kao et al. (2017) studied this question.

synapsesocial.com/papers/6a9bd75cc4ca1e4d5c68ea9e https://doi.org/10.1002/lary.26499

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Implication

Preclinical study reveals surface charge modification decreases Pseudomonas aeruginosa adherence and implant colonization in rats, suggesting a strategy to prevent device infections.

Surface charge modification decreases Pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model

Discussion

Member takes

Implication

Key Points

Cite This Study

Surface charge modification decreases Pseudomonas aeruginosa adherence in vitro and bacterial persistence in an in vivo implant model

Discussion

Member takes

Implication

Key Points

Cite This Study

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