Supplementary Figure 10 from Targeting the ATF6-mediated ER stress response and autophagy blocks integrin-driven prostate cancer progression

Figure S10. (A) Evaluation of MGAT5-mediated Integrin αv glycosylation in the orthotopic tumor samples. Tissue samples were co-stained with Integrin αv (green) and Cy3 labeled PHA-L lectin (red). Representative Z-stack images were collected by SIM and reconstructed using Imaris for clarity. (B) Mander's colocalization between red and green in the samples from A. Kruskal-Wallis test; n indicates the number of foci counted. For all statistics: median ± SD, ****p<0.0001, ***p<0.001, **p<0.005, *p<0.05. (C) Evaluation of MGAT3-mediated Integrin αv glycosylation in the orthotopic tumor samples. Tissue samples were co-stained with Integrin αv (green) and Cy3 labeled PHA-E lectin (red). Representative Z-stack images were collected by SIM and reconstructed using Imaris for clarity; bars, 3 µm. (D) Mander's colocalization between red and green in the samples from C. Kruskal-Wallis test; n indicates the number of foci counted; mean ± SD, ****p<0.0001, **p<0.01, and *p<0.05.