Supplementary Figure S10 from Autocrine 17-β-estradiol/estrogen receptor-α loop determines the response to immune-checkpoint inhibitors in non-small cell lung cancer

Dose-dependent effects of fulvestrant and letrozole. ERα highNCI-H1975 cells were grown 24 h in fresh medium (Ctrl) or in medium containing 0.1, 1, 10 nM fluvestrant (Fulv, panel a), an ERα inhibitor, or 0.1, 1, 10 nM letrozole (Letr, panel b), an aromatase inhibitor. As read-out assays, the expression of ERα-target gene CXCL12 was measured by RT-PCR in triplicate (panel a), the amount of 17-β-estradiol was measured by ELISA (technical duplicate, panel b). The relative expression in Ctrl cells was considered 1. Data are means+SD (n=3, biological replicates). *p<0.05, ***p<0.001: treated cells versus Ctrl cells (ANOVA).