Supplementary Figure S11 from Autocrine 17-β-estradiol/estrogen receptor-α loop determines the response to immune-checkpoint inhibitors in non-small cell lung cancer

Activity of ERβ on CD274/PD-L1 promoter. Chromatin immunoprecipitation of ERE1 and ERE2 regions from CD274/PD-L1 promoter, using an anti-ERα or an anti-ERβ antibody, followed by RT-PCR (technical triplicates) of the precipitated products, in ERα highNCI-H1975 cells, grown 24 h in fresh medium (Ctrl) or in medium containing 10 nM fluvestrant (Fulv), an ERα inhibitor, or 10 nM letrozole (Letr), an aromatase inhibitor. Data are means+SD (n=3). **p<0.01, ***p<0.001: treated cells versus Ctrl cells; °°°p<0.001: anti-ERβ versus anti-ERα.