Supplementary Figure S9 from Autocrine 17-β-estradiol/estrogen receptor-α loop determines the response to immune-checkpoint inhibitors in non-small cell lung cancer

PD-L1 inhibition does not affect amount and phosphorylation of estrogen receptor α. Female-derived ERα lowNCI-H1385 and ERα highNCI-H1975 cells, male-derived ERα lowA549 and ERα highNCI-H1650 cells were treated 24 h in the absence (-) or presence (+) of 1 μg/mL atezolizumab (Atezo), a PD-L1 inhibitor, then lysed and analyzed for the indicated proteins in nuclear extracts. TBP is included as control of equal protein loading. The image is representative of one of three experiments.