Supplementary Figure S5 from Autocrine 17-β-estradiol/estrogen receptor-α loop determines the response to immune-checkpoint inhibitors in non-small cell lung cancer

Disaggregated data of ERα, 17-β-estradiol and PD-L1 levels in non-small cell lung cancer cells. a-c. Expression of ERα mRNA, measured by RT-PCR (technical triplicates) (panel a), levels of 17-β-estradiol, measured by ELISA (technical triplicates) (panel b), expression of surface PD-L1 (panel c), measured by flow cytometry (technical triplicates) in 29 human NSCLC cell lines, divided in female (F)- or male (M)-derived cell lines, primary tumor (P) or metastatic (MTS) localization-derived cell lines, non-smoker (NSm) or smoker (Sm) patients. *p<0.05, **p<0.01: F versus M, P versus MTS, NSm versus Sm (ANOVA).