Supplementary Figure S4 from Autocrine 17-β-estradiol/estrogen receptor-α loop determines the response to immune-checkpoint inhibitors in non-small cell lung cancer

Disaggregated data of PD-L1 and ERα in non-small cell lung cancer patients, according to ages, tumor stages and smoking habits. Expression of PD-L1 (panel a) and ERα (panel b) mRNA, measured by RT-PCR (technical triplicates) in tumor samples of 35 NSCLC patients, according to pathological classification (T: primary tumors; N: lymph nodes involved; M: metastasis), clinical stages (III-IV stage), smoking habits (never smoker: NSm; former smoker: FSm; current smoker: CSm). *p<0.05, **p<0.01, ***p<0.001: T versus N+M, III vs IV, NSM+FSm vs CSm (ANOVA).