Preparation of Extra Cellular Matrix Scaffold from Bovine Amniotic Membrane

Decellularization is a critical process in tissue engineering, aimed at removing cellular components from tissues while preserving the extracellular matrix (ECM). The bovine amniotic membrane (BAM) has gained interest due to its rich ECM composition and potential applications in regenerative medicine. This study, based on the work of Ballesteros et al. (2020) with a modification, explores the decellularization of BAM using chemical and physical method to remove cellular content while preserving the ECM structure and composition. The success of decellularization was evaluated through histological staining. Results demonstrated efficient decellularization with minimal impact on the ECM structure, making BAM a promising scaffold for tissue engineering.