This protocol is designed for detecting choline acetyltransferase (ChAT) using the Millipore AB144P (RRID:AB_2079751) and human alpha-synuclein (Abcam, RRID:AB_2537217, MJFR1). Tissues stained with this protocol include 35 μm free-floating mouse brain sections. All tissues were from mice perfused with 4% PFA.
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Pietro La Vitola (2024) studied this question.
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