The Efficacy of Desmopressin and Imipramine in Retaining the Instilled Suspension in the Bladder

OBJECTIVE: To assess the efficacy of desmopressin and imipramine in reducing urination frequency, and retaining the instilled suspension in the bladder without maintaining the mouse under anesthesia. METHOD: Twenty-four mice were divided into four groups: (1) control, (2) four-hour water deprivation, (3) two µg/kg intraperitoneal desmopressin, and (4) two µg/kg intraperitoneal desmopressin plus 30 mg/kg imipramine gavage. Micturition frequency was recorded using voiding spot assay. Animals were catheterized and 50 µl of methylene blue was instilled into the bladder. The mice were then placed on a white paper to recover. Blue voiding was documented. RESULTS: Urination frequency was significantly lower in the desmopressin group. The water-deprived and desmopressin/imipramine-combination groups did not differ significantly compared to control group. All animals voided methylene blue upon recovery. CONCLUSION: Desmopressin notably reduced urination frequency. In contrast, water deprivation did not do so. Desmopressin and imipramine failed to cause suspension retention in awake mice.