An Integrated Assessment of Different Depositional Paleoenvironment Using Nitrogen Markers and Biomarkers after Chromatographic Methods Optimization

In this study, chromatographic methods were first optimized to ensure the robustness of the identification and quantification of nitrogen markers and biomarkers. Then, the optimal conditions were applied to 14 crude oil samples deposited in distinct paleoenvironments from Brazil, Venezuela, and Colombia to perform an integrated geochemical assessment. Analytical standards, certified reference material, and retention indices were used to confirm the identification of biomarkers and N-markers. The results of geochemical interpretations based on unresolved complex mixture (UCM), pristane/