Figure 3 from Poor Prognosis among Radiation-Associated Bladder Cancer Is Defined by Clinicogenomic Features

Identification of de novo mutational signatures. A, Heatmap of the cosine similarity between the de novo mutational signatures and known Catalogue of Somatic Mutations in Cancer SBS profiles. Boxplots of the signature contribution to the overall SNVs for each sample grouped by (B) signature and latency or (C) smoking status and signature for CTRL and (D) RT tumors. Pairwise comparisons between CTRL (gold), RT–short latency (dark blue), and RT–long latency (light blue) tumors were made using the Wilcoxon rank-sum test, with P values listed above their corresponding comparison. Boxplots are represented by the IQR and midline at the median. Error bars equal the Q1/Q3 ± 1.5 × IQR. E, Samples were grouped by the mutational signature with the greatest signature contribution. KM curves were generated based on this max signature assignment for OS for RT patients. Dashed lines indicate median survival. G, Forest plot visualizing a multivariate Cox proportional model for OS incorporating the max signature group, latency, and smoking status. WT, wild type.