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Abstract The aim of this study was to evaluate the influence of a Bacillus-based probiotic supplementation on intake, digestibility, and ruminal fermentation parameters of beef cows consuming a low-quality forage diet (7.0% CP, 60% NDF, 39% ADF, DM basis). Ruminally fistulated Angus × Hereford cows n = 15; age = 6.1 ± 1 yr; body weight (BW) = 655 ± 57 were used in two identical complete randomized designs, housed in individual pens with water, and forage intake monitored daily. Treatments consisted of 1) 500 g of protein supplement (dry distillers grain + mineral mix) without probiotic (CON n = 15), and 2) 500 g of protein supplement + 3 g of a probiotic containing Bacillus licheniformis and B. subtilis (BOV; n = 15; 2.2 ×109 CFU of the mixture/g; Bovacillus, Novonesis, Hørsholm, Denmark). Each experimental period lasted 33 d, consisting of 21 d of adaptation, 12 d of collection, and 30 d of wash-out between periods. From d 22 to 26, fecal samples were collected to determine intake and nutrient digestibility using titanium dioxide as an indigestible marker. On d 27, ruminal fluid was collected at h 0 (before feeding), 4, 8, 12, 16, and 20 post-feeding to evaluate short-chain fatty acid (SCFA) and N-NH3 concentration. From d 28 to 32, hay samples (4 g) were fitted in Dracon bags and incubated in the rumen for 0, 12, 24, 48, 72, 96, and 120 h to determine nutrient digestibility. Cows receiving BOV had greater (P 0.001) dry matter intake compared with the CON group (Table 1). Also, cows supplemented with BOV had greater (P 0.02) dry matter and protein digestibility and a tendency (P ≤ 0.07) for greater NDF and ADF forage digestibility compared with CON. A tendency was also detected (P = 0.09) for the degradability of forage dry matter, which was improved in BOV vs. CON. No effects were observed on rumen fermentation characteristics (P ≥ 0.14; Table 2).). In conclusion, adding bacillus-based probiotics enhanced the intake, nutritional digestibility, and degradability of beef cows fed medium-quality forage diets.
Marques et al. (Sun,) studied this question.