GC-MS/MS-Based Detection and Quantification of Methamphetamine and Mephedrone in Biological Matrices using Modified dSPE Method

This research presents a technique combining gas chromatography and tandem mass spectrometry (GC-MS/MS) with a modified dispersive solid phase extraction (D-SPE) method for identifying and quantifying methamphetamine (MTPn) and mephedrone (MPDn) in biological samples. These amphetamine-category new psychoactive substances are increasingly popular among young individuals, making their detection crucial. The method involves a clean-up procedure integrated with GC-MS/MS technology, allowing simultaneous detection of both drugs. Operating the analytical column at 40 ºC with sample concentrations ranging from 5 to 200 ng/mL, the study determined the limit of detection (7.326-20.213 ng/mL) and the limit of quantification (22.2-60.25 ng/mL). The modified dSPE method’s efficacy was compared to other extraction techniques, highlighting the sensitivity, accuracy and precision of the GC-MS/MS method. These findings have significant implications for forensic and clinical applications in detecting and quantifying methamphetamine and mephedrone.