Evaluation of Bond Strength of a Bioactive Adhesive System Associated with Ozonized Water on Enamel Preconditioned with Phosphoric Acid

Aims: To evaluate the effectiveness of ozonated water as a cavity cleaning solution in combination with a bioactive adhesive system on bond strength to enamel, compared to distilled water, after different storage times. Study Design: Experimental in vitro study. Place and Duration of Study: Department of Restorative Dentistry, State University of West Parana, Cascavel, Parana, Brazil, between September 2022 and August 2023. Methodology: Bovine anterior teeth were divided into four groups: AD24h (distilled water and 24-hour storage), AO24h (ozonated water and 24-hour storage), AD30D (distilled water and 30-day storage), and AO30D (ozonated water and 30-day storage). After cavity cleaning, a bioactive adhesive system and composite resin were applied. Three bioactive composite resin cylinders were light-cured on each tooth using a LED light device. Microshear bond strength was tested with a testing machine (EMIC) at a speed of 1 mm/min using a 50N load cell. Statistical analysis was performed using the non-parametric Wilcoxon test (p < 0.05). Results: Ozonated water significantly increased bond strength to enamel after 24 hours (p < 0.05). However, after 30 days, no significant differences in bond strength were observed between ozonated water and distilled water groups. Conclusion: Ozonated water enhances initial bond strength to enamel but its effect diminishes over time. These findings suggest the need for improved adhesion techniques to achieve long-term durability in dental restorations.