This protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining for Tyrosine Hydroxilase (TH) of free-floating fixed brain tissue sections using the avidin/biotin ABC complex and the Rabbit Polyclonal Anti-TH NB300-109 (Novus). This protocol has been tested with free-floating non-human primate (NHP) brain tissue that has been fixed (10% formalin or 4% paraformaldehyde), cryoprotected (sucrose or glycerol gradients), and cryo-sectioned .
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Andreea C. Bostan (2024) studied this question.