Establishing the Approach to the Diagnosis of Hemolytic Anemia in the Genetic Era: A Case Series

Hemolytic anemia is characterized by the premature destruction of red blood cells, a condition that ranges from chronic to life-threatening. Hereditary hemolytic anemias (HHAs) encompass a broad spectrum of disorders including hemoglobinopathies, enzymopathies, and membrane disorders. In India, hemoglobinopathies, notably thalassemia and sickle cell disease, are significant health concerns contributing to high morbidity and mortality rates. Despite many cases being clinically insignificant, these disorders exert a considerable public health burden due to their prevalence. Techniques like next-generation sequencing (NGS) and high-performance liquid chromatography (HPLC) have emerged as powerful tools for identifying and diagnosing HHAs. NGS enables comprehensive genetic analysis, pinpointing mutations associated with hemoglobinopathies and other forms of hereditary anemia. HPLC allows precise quantification and characterization of hemoglobin variants, which is crucial for diagnosing hemoglobinopathies.