Abstract α-enolase (ENO1), as a plasminogen receptor and a key enzyme of glycolysis, is highly expressed in cervical cancer, Inhibition of ENO1 activity can further inhibit the migration and invasion of tumor cells by inhibiting glycolsis, and inhibiting tumor growth.In this study, adeno-associated virus (rAAV-ENO1mAb-5) with the variable region gene of recombinant ENO1 monoclonal antibody was prepared to mediate the targeted entry of the variable region gene of recombinant ENO1 monoclonal antibody into the cytoplasm to observe its growth inhibition effect on cervical cancer cells. The results showed that the variable region gene of rAAV-ENO1mAb-5, which was highly effective and targeted to load ENO1, entered the cytoplasm and inhibited the proliferation and migration of cervical cancer cells by antagonizing ENO1 enzyme activity, significantly reducing lactate and pyruvate content (P < 0.01). In conclusion, rAAV-ENO1mAb-5 can specifically block ENO1 expression on cell membrane and inhibit ENO1 glycolysis activity in tumor cells, which has therapeutic effect on cervical cancer cells.
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Yang et al. (2024) studied this question.
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