Effect of Accelerated Storage on the Stability of Azoxystrobin Fungicide and It Is Toxicity on Albino Rats

Objective: This study was conducted to determined the effect accelerated storage procedure on fungicide stability, as well as, to evaluate the impact of sub lethal doses of tested fungicides on reproductive and liver in male albino rats. Methods: Determine the effect of accelerated storage at 54±2℃ for 3, 7, 14 and 21 days on the stability of azoxystrobin. Investigate the effects of sub chronic exposure on male albino rats. Investigate the effects of sub chronic exposure on histopathological examination of the liver. Results: Azoxystrobin were relatively stable after accelerated storage at 54±2℃ for 21 days. The mediam lethal dose (LD50) of azoxystrobin was 681.71mg/kg. Azoxytrobin 25% induced a significantly decreased in the sperm count and the motility percent at 1/20 and 1/40 LD50.The serum LH and FSH levels decreased with increasing doses of azoxystrobin. There were no histopathological alteration occurred after treated with azoxystrobin at 1/20 and 1/40 of LD50. The ALT and AST enzymes levels increased to (7.75 &.36.58U/L) and (4.39 &15.23U/L) after treated with 1/20 and 1/40 of LD50, also, the cholesterol and triglycerides levels were increased recoding (116.01 &184.89mg/dL) and (110.18 &138.24mg/dL) after treated with 1/20 and 1/40 of LD50, respectively. The rats treated with 1/20 of LD50 displayed massive inflammatory cells aggregation. Conclusion: Azoxystrobin were relatively stable after accelerated storage at 54±2℃ for 21 days. The high dose 1/20 of LD50 decreased the serum LH and FSH levels, increased the liver enzymes, and induced histopathological effects of the liver.