Figure 1 from Tumoral Interferon Beta Induces an Immune-Stimulatory Phenotype in Tumor-Associated Macrophages in Melanoma Brain Metastases

Induction of tumoral IFNβ impairs intracranial YUMM5.2 Ifnb1eGFP tumor growth. A, YUMM5.2 Ifnb1eGFP and YUMM5.2 eGFP control cells were treated with 1 μg/mL Dox or left untreated for 48 hours before cell culture supernatant was harvested. IFNβ concentrations were analyzed by ELISA and are displayed in pg/mL (n = 3). B, YUMM5.2 Ifnb1eGFP cells were treated with 1 μg/mL Dox and cell culture supernatant was harvested after 24, 48, and 72 hours. IFNβ concentrations were analyzed by ELISA and are displayed in pg/mL (n = 3). C–G, C57Bl/6J mice received control diet or Dox diet from day 1 on following intracranial YUMM5.2 Ifnb1eGFP tumor cell inoculation. Tumor growth was monitored by MRI on day 14, tumors were excised and blood was drawn on day 16. C, Schematic timeline. D, Intratumoral IFNβ concentrations were analyzed by ELISA and are displayed in pg/mL of original tumor volume (n = 6 for each group). E, Plasma IFNβ concentrations were analyzed by ELISA and are displayed in pg/mL. (n = 6 for each group). F, Tumor volume as measured by MRI is displayed in μL (T1 sequence; n = 8 for each group). G, Representative coronal MR images in T1 sequence with contrast agent. Each image represents one mouse from the indicated treatment group. Data are expressed as median + IQR and individual values for A, B, and D–F. Statistical significance was determined by Mann–Whitney U test for A, B, and D–F. ES, effect size. (C, Created with BioRender.com.)