Quantification of pathological protein accumulation (aSyn and tau) in transplanted human iPSC-derived dopamine neurons v1

This protocol details the immunohistochemistry protocol and subsequent imaging analysis protocols for quantifying pathological protein accumulation in human derived iPSC dopaminergic neurons transplanted into athymic mice. Tissue sections were stained free floating for human dopamine cells (tyrosine hydroxylase antibody) and pathological markers (Table 1) as per protocol.