The Mechanism of Cistanche Yishen Granules in Treating Parkinson's Disease Based on Network Pharmacology and Molecular Docking

Abstract To evaluate the potential pharmacological effects of bioactive components in Citanche Yishen Granules (CYSG) on Parkinson's disease (PD) using network pharmacology methods. Method: The effective active ingredients in CYSG were retrieved using the TCMSP database, and the potential gene targets of these active ingredients were predicted using the Swiss Target Prediction database. The PD gene was obtained from three databases. Then, we intersect the targets of CYSG active ingredients with PD targets. At the same time, these common targets were subjected to Drug-active ingredient-common target network construction, protein-protein interaction (PPI) analysis, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Finally, molecular docking studies further confirmed the results of these analyses. Result: CYSG has 68 active ingredients that meet the screening criteria. These 68 active ingredients have a total of 799 potential gene targets, of which 635 are shared with PD. The biological process (BP) in GO enrichment analysis of these common targets includes protein autophotography, peptide tyrosine pharmacology, and peptide tyrosine modification. The Cellular Component (CC) mainly includes the membrane raft, membrane microdomain, and membrane region. The molecular function (MF) mainly includes protein tyrosine kinase activity, protein serine/threonine kinase activity, and neuron receptor activity. The KEGG pathway mainly includes related pathways such as Neuroactive light receiver interaction, Calcium signaling pathway, Lipid and atherosclerosis and Apoptosis. The molecular docking results showed that the top three active components of CYSG had good binding activity with four core targets. In summary, this study provides a deep understanding of the potential pharmacological effects of the active components of CYSG on PD, supporting it as the most promising treatment method for this disease.