Ultra-low-field brain MRI morphometry: test-retest reliability and correspondence to high-field MRI

Magnetic resonance imaging (MRI) enables non-invasive monitoring of healthy brain development and disease. Widely used higher field (>1.5 T) MRI systems are associated with high energy and infrastructure requirements, and high costs. Recent ultra-low-field (<0.1T) systems provide a more accessible and cost-effective alternative. However, it is not known whether anatomical ultra-low-field neuroimaging can be used to extract quantitative measures of brain morphometry, and to what extent such measures correspond to high-field MRI. Here we scanned 23 healthy adults aged 20-69 years on two identical 64 mT systems and a 3 T system, using T 1 w and T 2 w scans across a range of (64 mT) resolutions. We segmented brain images into 4 global tissue types and 98 local structures, and systematically evaluated between-scanner reliability of 64 mT morphometry and correspondence to 3 T measurements, using correlations of tissue volume and Dice spatial overlap of segmentations. We report high 64 mT reliability and correspondence to 3 T across 64 mT scan contrasts and resolutions, with highest performance shown by combining three T 2 w scans with low through-plane resolution into a single higher-resolution scan using multi-resolution registration. Larger structures show higher 64 mT reliability and correspondence to 3 T. Finally, we showcase the potential of ultra-low-field MRI for mapping neuroanatomical changes across the lifespan, and monitoring brain structures relevant to neurological disorders. Raw images and code are publicly available ( upon publication ), enabling systematic validation of pre-processing and analysis approaches for ultra-low-field neuroimaging.