The effectiveness of some biocontrol agents (Pseudomonas fluorescens & Trichoderma viride) & organic acids (acetic acid & boric acid) against soft rot bacteria Pectobacterium sp. were studied under laboratory & field conditions. Two soft rot bacterial isolates Pectobacterium carotovorum subsp. carotovorum Pcc & Pectobacterium carotovorum subsp. carotovorum Pcc160 (Acc. No. LN811442) were used in this study. Pcc isolate was isolated from Potato tuber (Burn variety), Pcc & Pcc160 isolates caused soft rot symptoms of potato. PCR was used to identify the Pcc isolate molecularly using universal primers for 16S rRNA. The PCR products' DNA sequence, BLAST analysis, & Genbank data revealed that the Pcc isolate belonged to Pectobacterium carotovorum sub sp. carotovorum. According to the phylogenetic tree based on the DNA nucleotide sequences of the 16s rRNA gene, the Egyptian isolate resembles the Chinese isolate (OQ727369) closely. In vitro acetic acid showed the strongest effect against Pcc & Pcc160 isolates with inhibition zones 41.33 & 33.67 mm & MIC 50 & 100 μg/mL respectively.
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Hanan Farouk Badri Youssef (2024) studied this question.
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