Fabrication of Aptasensor for Monitoring cancer biomarkers: Detection of Lung Specific Biomarkers Using Chemical Converted Graphene

Abstract This work was focused on development a stable, selective and sensitive electrochemical aptasensor based on 3,4,9,10-perylene tetracarboxylic acid -functionalized chemical converted graphene (PTCA/CCG) modified electrode for monitoring neuron specific enolase (NSE), lung-specific biomarkers. The electrochemical aptasensor was relied on the purified NSE aptamer immobilized on PTCA/CCG modified electrode. The PTCA/CCG promoted both the immobilization of the aptamer and the electrochemical signal. The aptasensor exhibited a low limit of detection (0.008 ng/mL), broad linear range (10⁻ ³ to 10³ ng/mL), remarkable specificity, and excellent repeatability and long-term stability, maintaining initial resistance at 96.57% after 30 days. Results exhibited that the recoveries ranging from 98.00–99.00% and relative standard deviation less than 4.08%, demonstrating the acceptable precision and reliability of the fabricated aptadensor and confirming the aptasensor's performance and efficiency in complex biological fluids and clinical applications.